Fig. 2. GALNT14 is a target gene of Osx. (a) Heatmap representing the expression levels of differential expressed genes between 231-OE6 and in 231-OEC cells. The red and green colours indicate the up- and down-regulated genes, respectively. (b) Partially upregulated genes in 231-OE6 were demonstrated by qRT-PCR. β-Actin was used as an internal control. (c) Cell lysates from 231- KD2, 231-KDC, 231-OE6, and 231-OEC cells were immunoblotted with anti-GALNT14 and anti-Osx antibodies. β-Actin served as a loading control. The 64-kDa blot of GALNT14 and 45-kDa blot of Osx were cropped from the same gel and were run under the same experimental conditions. The fold change of KD or OE vs. control was calculated after quantification and was presented above each blot. (d) Expression of Osx and GALNT14 in breast carcinoma sections from a nude mouse model established by the injection of 231-OE6, 231-KD2 and their corresponding control cells into the mammary fat pad of female nude mice, as detected by the IHC assay. (e) Expression of Osx and GALNT14 in human breast cancer tissues, as detected by the IHC assay.